Search Results for author: Daniel Schindler

Found 6 papers, 0 papers with code

Use of a Golden Gate plasmid set enabling scarless MoClo-compatible transcription unit assembly

no code implementations5 Feb 2024 Stijn T. de Vries, Laura Kley, Daniel Schindler

This feature has been used to define hierarchical Golden Gate assembly standards with defined overhangs ("fusion sites") for defined part libraries.

Validation of Golden Gate assemblies using highly multiplexed Nanopore amplicon sequencing

no code implementations25 Jan 2024 Adan A. Ramirez Rojas, Cedric K. Brinkmann, Daniel Schindler

The workflow, called DuBA. flow, is a start-to-finish procedure that provides all necessary steps from a single colony to the final easy-to-interpret sequencing report.

In silico design, in vitro construction and in vivo application of synthetic small regulatory RNAs in bacteria

no code implementations28 Apr 2023 Michel Brueck, Bork A. Berghoff, Daniel Schindler

This protocol outlines how to use computational predictions and synthetic biology approaches to design, construct and validate synthetic sRNA functionality for their application in bacteria.

Three-component contour dynamics model to simulate and analyze amoeboid cell motility

no code implementations24 Oct 2022 Daniel Schindler, Ted Moldenhawer, Carsten Beta, Wilhelm Huisinga, Matthias Holschneider

The model is used to generate contour data for a variety of qualitatively different, e. g., polarized and non-polarized, cell tracks that are hardly distinguishable from experimental data.

Analysis of protrusion dynamics in amoeboid cell motility by means of regularized contour flows

no code implementations26 May 2020 Daniel Schindler, Ted Moldenhawer, Maike Stange, Valentino Lepro, Carsten Beta, Matthias Holschneider, Wilhelm Huisinga

Amoeboid cell motility is essential for a wide range of biological processes including wound healing, embryonic morphogenesis, and cancer metastasis.

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