BioDrone is the first bionic drone-based single object tracking benchmark, it features videos captured from a flapping-wing UAV system with a major camera shake due to its aerodynamics. BioDrone highlights the tracking of tiny targets with drastic changes between consecutive frames, providing a new robust vision benchmark for SOT. 1. Large-scale and high-quality benchmark with robust vision challenges 2. Rich challenging factor annotation 3. Videos from Bionic-based UAV 4. Tracking baselines with comprehensive experimental analyses